Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Usage Physical State: Freeze-dried solidStorage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ConjugateHorseradish Peroxidase
Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Images & ReferencesThis product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
胶原酶
Cat＃5002-重组人类MMP-13（截短）-待定，直至将来通知。对于给您带来的不便，我们深表歉意。
胶原蛋白是基质金属蛋白酶（MMP）家族的成员，基质金属蛋白酶是一组蛋白酶，参与细胞外基质（ECM）和结缔组织的生理和病理转换。MMP可以根据其报告的底物特异性（胶原蛋白，明胶酶，基质溶菌素）和结构（膜型MMP）进行分组。胶原蛋白（MMP-1，MMP-8，MMP-13）可以在其定型三螺旋结构域中特异性降解天然纤维状胶原蛋白（I，II，III，VII和X型），以及变性胶原蛋白（明胶）。然而，底物特异性在胶原酶之间变化。胶原酶的表达在健康组织中通常较低，但在炎性条件，恶性组织和其他产生促炎性细胞因子环境的病理条件下升高。因此，胶原酶是影响软骨组织的疾病的诱人治疗靶标。
为了研究针对天然形式I型胶原和II型胶原底物的蛋白酶活性，Chondrex，Inc.提供了快速胶原酶活性测定试剂盒以及 细菌胶原酶活性测定试剂盒。通过使用天然形式的胶原蛋白作为底物，这些测定法可以排除其他MMP的酶促活性，因为只有胶原酶才能切割天然形式的原纤维胶原蛋白。此外，MMP-13抑制剂活性测定试剂盒适用于评估MMP-13抑制剂的功效。
Chondrex，Inc.还提供全长，潜伏形式的重组人MMP-8（Cat＃5001）以及截短的重组人MMP-13（Cat＃5002），它们是使用I型胶原蛋白进行胶原酶活性测定的理想参考（Cat＃4001）和II型胶原蛋白（Cat＃4002）基材。
要了解有关我们各种胶原酶/ MMP活性测定试剂盒，MMP酶和胶原酶底物的更多信息，请继续阅读以下内容。
胶原酶检测试剂盒
| 产品 | 目录 ＃ | 价格（美元） |
| 细菌胶原酶测定试剂盒 | 3014 | 375.00 |
| MMP-13抑制剂检测试剂盒 | 3003 | 510.00 |
| 具有I型胶原底物的快速胶原酶测定试剂盒 | 3001 | 467.00 |
| 具有II型胶原底物的快速胶原酶测定试剂盒 | 3002 | 467.00 |
胶原酶底物
| 产品 | 目录 ＃ | 价格（美元） |
| FITC标签的牛I型胶原蛋白底物 | 4001 | 199.00 |
| FITC标签的牛II型胶原蛋白底物 | 4002 | 244.00 |
胶原蛋白（MMP）
| 产品 | 目录 ＃ | 价格（美元） |
| 重组人类MMP-13（截短） | 5002 | 270.00 |
| 重组人类MMP-8（全长，潜伏形式） | 5001 | 270.00 |
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